Publications - Pushkar Lele Lab

Key Works

J. D. Antani, R. Gupta, A. H. Lee, K. Y. Rhee, M. D. Manson & P. P. Lele, “Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.” Nature Communications (2021)
J. Antani, A. X Sumali, T. P. Lele, P.P. Lele, “Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori”, ELife (2021)
Rachit Gupta, Kathy Y. Rhee, Sarah D. Beagle, Ravi Chawla, Nicolas Perdomo, Steve W. Lockless and Pushkar P. Lele, “Indole modulates cooperative protein-protein interactions in the flagellar motor”, PNAS Nexus (2022)
R. Chawla, R. Gupta, T. P. Lele, P. P. Lele, “A skeptic’s guide to bacterial mechanosensing”, Journal of Molecular Biology, (2020), 432 (2), 523
J. Yang, R. Chawla, K. Rhee, R. Gupta, M. Manson, A. Jayaraman, P. P. Lele, “Biphasic chemotaxis of Escherichia coli to the microbiota metabolite indole”, Proceedings of the National Academy of Sciences, USA , (2020), 117 (11) 6114-6120
P.P. Lele, T. Roland, A. Shrivastava, Y. Chen, H. C. Berg, “The flagellar motor of Caulobacter crescentus generates more torque when a cell swims backward.”, Nature Physics (2016), 12, 175-178.
P.P. Lele, A. Shrivastava, T. Roland, H. C. Berg, “Response thresholds in bacterial chemotaxis”, Science Advances (2015), 1:e150029ss.
P. P. Lele, Basarab G. Hosu, H. C. Berg, “Dynamics of mechanosensing in the bacterial flagellar motor”, Proceedings of the National of Academy of Sciences, USA (2013), 110 (29), 11839-11844.
P.P. Lele, R. W. Branch, V. Nathan, H. C. Berg, “Mechanism for adaptive remodeling of the bacterial flagellar switch”, Proceedings of the National of Academy of Sciences, USA (2012), 109 (49), 20018-20022.

Full List

P. Shanmughan, P. Subrahmaniyan, D. Bhatnagar, S. Ranganathan, P. P. Lele, “Urea-loaded PLGA microspheres as point-sources of attractants for Helicobacter pylori”, Biotechnology and Bioengineering, accepted.
S. Mukhopadhyay, R. Bishayi, A. Shaji, A. H. Lee, R. Gupta, M. Mohajeri, A. Katiyar, B. McKee, I. R. Schmitz, R. Shin, T. P. Lele, P. P. Lele, “Dynamic Adaptation in Extant Porins Facilitates Antibiotic Tolerance in Energetic Escherichia coli“, under review
T. Wang, S. Abolghasemzade, B. P. McKee, I. Singh, K. Pendyala, M. Mohajeri, H. Patel, A. Shaji, A. L. Kersey, K. Harsh, S. Kaur, C. R. Dollahon, S. Chukkapalli, P. P. Lele, D. E. Conway, A. K. Gaharwar, R. B. Dickinson, and T. P. Lele, “Matrix stiffness drives drop-like nuclear deformation and lamin A/C dependent YAP nuclear translocation”, Nature Communications, accepted
J. D. Antani, A. Shaji, R. Gupta, P. P. Lele, “Reassessing the Standard Chemotaxis Framework for Understanding Biased Migration in Helicobacter pylori“, Annual Review of Chemical and Biomolecular Engineering, 2023.
N. A. Vidwans, K. Rhee, P. P. Lele, S. Vaddiraju, “Real-time Deduction of Mechanisms and Kinetics Underlying Photocatalytic Water Disinfection: Cell Motility and Particle Tracking”, ACS ES&T Water (2023)
J. Yang, R. Chawla, K. Y. Rhee, R. Gupta, A. Aditya, P. Rane, M. D. Manson, A. Jayaraman, P. P. Lele, “Indole is sensed by multiple receptor domains in Tar and Tsr”, under review
A. H. Lee, R. Gupta, H. N. Nguyen, I. R. Schmitz, D. A. Siegele, and P. P. Lele, “Heterogeneous Distribution of Proton-Motive Force in Nonheritable Antibiotic Resistance”. mBio (2023)
Editorial: M. D. Manson, B. Nan, P. P. Lele, J. Liu and T. M. Duncan, “Editorial: Biological rotary nanomotors”. Front. Microbiol (2022)
A. Katiyar, J. Zhang, J. D. Antani, Y. Yu , P. P. Lele, C. A. Reinhart-King, N. J. Sniadecki, K. J. Roux, R. B. Dickinson, T. P. Lele, “The nucleus bypasses obstacles by deforming like a drop with surface tension mediated by lamin A/C”, Advanced Science (2022)
Rachit Gupta, Junhua Yuan and Pushkar P. Lele, “Bacterial proprioception: can the bacterium sense its motility?”, Front. Microbiol (2022)
Rachit Gupta, Kathy Y. Rhee, Sarah D. Beagle, Ravi Chawla, Nicolas Perdomo, Steve W. Lockless and Pushkar P. Lele, “Indole modulates cooperative protein-protein interactions in the flagellar motor”, PNAS Nexus (2022)
J. Antani & P. P. Lele, “Flagellar Sensing of Surfaces”, Physical Biology (2021)
J. D. Antani, R. Gupta, A. H. Lee, K. Y. Rhee, M. D. Manson & P. P. Lele, “Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.” Nature Communications (2021)
J. Antani, A. X Sumali, T. P. Lele, P.P. Lele, “Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori”, ELife (2021)
J. Yang, R. Chawla, K. Rhee, R. Gupta, M. Manson, A. Jayaraman, P. P. Lele, “Biphasic chemotaxis of Escherichia coli to the microbiota metabolite indole”, Proceedings of the National Academy of Sciences, USA , (2020), 117 (11) 6114-6120
A. Katiyar‡, J. D. Antani‡, B. P. McKee, R. Gupta, P. P. Lele, T. P. Lele, “A method for direct imaging of x–z cross‐sections of fluorescent samples”,Journal of Microscopy, (2020)
K Rhee, R. Chawla, P. P. Lele*, “Protein expression-independent response of intensity-based pH-sensitive fluorophores in Escherichia coli”, PLOS ONE , (2020), 15(6): e0234849
A. Orr, J. Yang, R. Chawla, K. Hull, M. Zhu, D. Romo, P. P. Lele, A. Jayaraman, M. Manson, P Tamamis, “Molecular Mechanism for Attractant Signaling to DHMA by E. coli Tsr”, Biophysical Journal , (2020) 118 (2), 492
R. Chawla, R. Gupta, T. P. Lele, P. P. Lele, “A skeptic’s guide to bacterial mechanosensing”, Journal of Molecular Biology , (2020), 432 (2), 523
K. M. Ford, J. Antani, A. Nagarajan, M. M. Johnson, P. P. Lele, “Switching and Torque Generation in Swarming cells of E. coli”, Frontiers in Microbiology , (2018), 9, 2197
C. Diethmaier, R. Chawla, A. Canzoneri, D.B. Kearns, P.P. Lele, D. Dubnau, “Viscous drag on the flagellum activates Bacillus subtilis entry into the K-state“, Molecular Microbiology, (2017), 106(3), 367-380
Book Chapter: P.P. Lele, “Molecular modeling of Switch-Remodeling”, Methods in Microbiology (Clifton, N.J), (2018), 1729:347-352.
R. Chawla, K. M. Ford, P. P. Lele, “Torque, but not FliL, regulates mechanosensitive flagellar motor-function”, Scientific Reports (2017), 7, 5565.
K. M., Ford, R, Chawla, P. P. Lele, “Biophysical Characterization of Flagellar Motor Functions”, Journal of Visualized Experiments(2017), 119, e55240.
P.P. Lele, T. Roland, A. Shrivastava, Y. Chen, H. C. Berg, “The flagellar motor of Caulobacter crescentus generates more torque when a cell swims backward.”, Nature Physics (2016), 12, 175-178.
P.P. Lele, A. Shrivastava, T. Roland, H. C. Berg, “Response thresholds in bacterial chemotaxis”, Science Advances (2015), 1:e150029ss.
P.P. Lele & H. C. Berg, “Switching of Bacterial Flagellar Motors Triggered by Mutant FliG”, Biophysical Journal (2015), 108 (5), 1275-1280.
A. Shrivastava, P. P. Lele, H. C. Berg, “Gliding is driven by a rotary motor”, Current Biology (2015), 25 (3), 338-341.
J. Wu, N. Shekhar, P. P. Lele, T. P. Lele, “FRAP analysis: accounting for bleaching during image capture”, PLoS ONE (2012), 7(8): e42854.
P. P. Lele, Basarab G. Hosu, H. C. Berg, “Dynamics of mechanosensing in the bacterial flagellar motor”, Proceedings of the National of Academy of Sciences, USA (2013), 110 (29), 11839-11844.
P.P. Lele, R. W. Branch, V. Nathan, H. C. Berg, “Mechanism for adaptive remodeling of the bacterial flagellar switch”, Proceedings of the National of Academy of Sciences, USA (2012), 109 (49), 20018-20022.
P.P. Lele, J. W. Swan, J. F. Brady, N. J. Wagner, E. M. Furst, “Colloidal diffusion and hydrodynamic screening near boundaries”, Soft Matter (2011), 7, 6844-6852.
P.P. Lele, E. M. Furst, “Assemble-and-Stretch method for creating two- and three-dimensional structures of anisotropic particles,” Langmuir (2009), 25, 8875-8878.
J. P. Singh, P. P. Lele, et al, “One and two dimensional assembly of colloidal ellipsoids in AC electric fields”, Physical Review E (2009), 79, 050401-050404.
P.P. Lele, M. Mittal, E. M. Furst, “Anomalous particle rotation and resulting microstructure of colloids in AC electric fields,” Langmuir (2008), 24(22), 12842-12848.
R Mittal, P. P. Lele, E. W. Kaler, E. M. Furst, “Polarization and interactions of colloidal particles in AC electric fields”, Journal of Chemical Physics (2008), 129 (6), 064513-064519.

Key Works

J. D. Antani, R. Gupta, A. H. Lee, K. Y. Rhee, M. D. Manson & P. P. Lele, “Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.” Nature Communications (2021)

J. Antani, A. X Sumali, T. P. Lele, P.P. Lele, “Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori”, ELife (2021)

Rachit Gupta, Kathy Y. Rhee, Sarah D. Beagle, Ravi Chawla, Nicolas Perdomo, Steve W. Lockless and Pushkar P. Lele, “Indole modulates cooperative protein-protein interactions in the flagellar motor”, PNAS Nexus (2022)

R. Chawla, R. Gupta, T. P. Lele, P. P. Lele, “A skeptic’s guide to bacterial mechanosensing”, Journal of Molecular Biology, (2020), 432 (2), 523

J. Yang, R. Chawla, K. Rhee, R. Gupta, M. Manson, A. Jayaraman, P. P. Lele, “Biphasic chemotaxis of Escherichia coli to the microbiota metabolite indole”, Proceedings of the National Academy of Sciences, USA , (2020), 117 (11) 6114-6120

P.P. Lele, T. Roland, A. Shrivastava, Y. Chen, H. C. Berg, “The flagellar motor of Caulobacter crescentus generates more torque when a cell swims backward.”, Nature Physics (2016), 12, 175-178.

P.P. Lele, A. Shrivastava, T. Roland, H. C. Berg, “Response thresholds in bacterial chemotaxis”, Science Advances (2015), 1:e150029ss.

P. P. Lele, Basarab G. Hosu, H. C. Berg, “Dynamics of mechanosensing in the bacterial flagellar motor”, Proceedings of the National of Academy of Sciences, USA (2013), 110 (29), 11839-11844.

P.P. Lele, R. W. Branch, V. Nathan, H. C. Berg, “Mechanism for adaptive remodeling of the bacterial flagellar switch”, Proceedings of the National of Academy of Sciences, USA (2012), 109 (49), 20018-20022.

Full List

P. Shanmughan, P. Subrahmaniyan, D. Bhatnagar, S. Ranganathan, P. P. Lele, “Urea-loaded PLGA microspheres as point-sources of attractants for Helicobacter pylori”, Biotechnology and Bioengineering, accepted.

S. Mukhopadhyay, R. Bishayi, A. Shaji, A. H. Lee, R. Gupta, M. Mohajeri, A. Katiyar, B. McKee, I. R. Schmitz, R. Shin, T. P. Lele, P. P. Lele, “Dynamic Adaptation in Extant Porins Facilitates Antibiotic Tolerance in Energetic Escherichia coli“, under review

J. D. Antani, A. Shaji, R. Gupta, P. P. Lele, “Reassessing the Standard Chemotaxis Framework for Understanding Biased Migration in Helicobacter pylori“, Annual Review of Chemical and Biomolecular Engineering, 2023.

N. A. Vidwans, K. Rhee, P. P. Lele, S. Vaddiraju, “Real-time Deduction of Mechanisms and Kinetics Underlying Photocatalytic Water Disinfection: Cell Motility and Particle Tracking”, ACS ES&T Water (2023)

J. Yang, R. Chawla, K. Y. Rhee, R. Gupta, A. Aditya, P. Rane, M. D. Manson, A. Jayaraman, P. P. Lele, “Indole is sensed by multiple receptor domains in Tar and Tsr”, under review

A. H. Lee, R. Gupta, H. N. Nguyen, I. R. Schmitz, D. A. Siegele, and P. P. Lele, “Heterogeneous Distribution of Proton-Motive Force in Nonheritable Antibiotic Resistance”. mBio (2023)

Editorial: M. D. Manson, B. Nan, P. P. Lele, J. Liu and T. M. Duncan, “Editorial: Biological rotary nanomotors”. Front. Microbiol (2022)

A. Katiyar, J. Zhang, J. D. Antani, Y. Yu , P. P. Lele, C. A. Reinhart-King, N. J. Sniadecki, K. J. Roux, R. B. Dickinson, T. P. Lele, “The nucleus bypasses obstacles by deforming like a drop with surface tension mediated by lamin A/C”, Advanced Science (2022)

Rachit Gupta, Junhua Yuan and Pushkar P. Lele, “Bacterial proprioception: can the bacterium sense its motility?”, Front. Microbiol (2022)

Rachit Gupta, Kathy Y. Rhee, Sarah D. Beagle, Ravi Chawla, Nicolas Perdomo, Steve W. Lockless and Pushkar P. Lele, “Indole modulates cooperative protein-protein interactions in the flagellar motor”, PNAS Nexus (2022)

J. Antani & P. P. Lele, “Flagellar Sensing of Surfaces”, Physical Biology (2021)

J. D. Antani, R. Gupta, A. H. Lee, K. Y. Rhee, M. D. Manson & P. P. Lele, “Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.” Nature Communications (2021)

J. Antani, A. X Sumali, T. P. Lele, P.P. Lele, “Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori”, ELife (2021)

J. Yang, R. Chawla, K. Rhee, R. Gupta, M. Manson, A. Jayaraman, P. P. Lele, “Biphasic chemotaxis of Escherichia coli to the microbiota metabolite indole”, Proceedings of the National Academy of Sciences, USA , (2020), 117 (11) 6114-6120

A. Katiyar‡, J. D. Antani‡, B. P. McKee, R. Gupta, P. P. Lele, T. P. Lele, “A method for direct imaging of x–z cross‐sections of fluorescent samples”,Journal of Microscopy, (2020)

K Rhee, R. Chawla, P. P. Lele*, “Protein expression-independent response of intensity-based pH-sensitive fluorophores in Escherichia coli”, PLOS ONE , (2020), 15(6): e0234849

A. Orr, J. Yang, R. Chawla, K. Hull, M. Zhu, D. Romo, P. P. Lele, A. Jayaraman, M. Manson, P Tamamis, “Molecular Mechanism for Attractant Signaling to DHMA by E. coli Tsr”, Biophysical Journal , (2020) 118 (2), 492

R. Chawla, R. Gupta, T. P. Lele, P. P. Lele, “A skeptic’s guide to bacterial mechanosensing”, Journal of Molecular Biology , (2020), 432 (2), 523

K. M. Ford, J. Antani, A. Nagarajan, M. M. Johnson, P. P. Lele, “Switching and Torque Generation in Swarming cells of E. coli”, Frontiers in Microbiology , (2018), 9, 2197

C. Diethmaier, R. Chawla, A. Canzoneri, D.B. Kearns, P.P. Lele, D. Dubnau, “Viscous drag on the flagellum activates Bacillus subtilis entry into the K-state“, Molecular Microbiology, (2017), 106(3), 367-380

Book Chapter: P.P. Lele, “Molecular modeling of Switch-Remodeling”, Methods in Microbiology (Clifton, N.J), (2018), 1729:347-352.

R. Chawla, K. M. Ford, P. P. Lele, “Torque, but not FliL, regulates mechanosensitive flagellar motor-function”, Scientific Reports (2017), 7, 5565.

K. M., Ford, R, Chawla, P. P. Lele, “Biophysical Characterization of Flagellar Motor Functions”, Journal of Visualized Experiments(2017), 119, e55240.

P.P. Lele, T. Roland, A. Shrivastava, Y. Chen, H. C. Berg, “The flagellar motor of Caulobacter crescentus generates more torque when a cell swims backward.”, Nature Physics (2016), 12, 175-178.

P.P. Lele, A. Shrivastava, T. Roland, H. C. Berg, “Response thresholds in bacterial chemotaxis”, Science Advances (2015), 1:e150029ss.

P.P. Lele & H. C. Berg, “Switching of Bacterial Flagellar Motors Triggered by Mutant FliG”, Biophysical Journal (2015), 108 (5), 1275-1280.

A. Shrivastava, P. P. Lele, H. C. Berg, “Gliding is driven by a rotary motor”, Current Biology (2015), 25 (3), 338-341.

J. Wu, N. Shekhar, P. P. Lele, T. P. Lele, “FRAP analysis: accounting for bleaching during image capture”, PLoS ONE (2012), 7(8): e42854.

P. P. Lele, Basarab G. Hosu, H. C. Berg, “Dynamics of mechanosensing in the bacterial flagellar motor”, Proceedings of the National of Academy of Sciences, USA (2013), 110 (29), 11839-11844.

P.P. Lele, R. W. Branch, V. Nathan, H. C. Berg, “Mechanism for adaptive remodeling of the bacterial flagellar switch”, Proceedings of the National of Academy of Sciences, USA (2012), 109 (49), 20018-20022.

P.P. Lele, J. W. Swan, J. F. Brady, N. J. Wagner, E. M. Furst, “Colloidal diffusion and hydrodynamic screening near boundaries”, Soft Matter (2011), 7, 6844-6852.

P.P. Lele, E. M. Furst, “Assemble-and-Stretch method for creating two- and three-dimensional structures of anisotropic particles,” Langmuir (2009), 25, 8875-8878.

J. P. Singh, P. P. Lele, et al, “One and two dimensional assembly of colloidal ellipsoids in AC electric fields”, Physical Review E (2009), 79, 050401-050404.

P.P. Lele, M. Mittal, E. M. Furst, “Anomalous particle rotation and resulting microstructure of colloids in AC electric fields,” Langmuir (2008), 24(22), 12842-12848.

R Mittal, P. P. Lele, E. W. Kaler, E. M. Furst, “Polarization and interactions of colloidal particles in AC electric fields”, Journal of Chemical Physics (2008), 129 (6), 064513-064519.

K Rhee, R. Chawla, P. P. Lele, “Protein expression-independent response of intensity-based pH-sensitive fluorophores in Escherichia coli”, PLOS ONE* , (2020), 15(6): e0234849